Journal: European Journal of Medical Research
Article Title: HMGB1 as a biomarker for myeloproliferative neoplasm complicated with atherosclerosis
doi: 10.1186/s40001-025-02655-8
Figure Lengend Snippet: HMGB1 Expression is Upregulated in MPN. A The qPCR assay revealed the expression of HMGB1 mRNA expression in bone marrow mononuclear cells, normalized to GAPDH. *** P < 0.0001 vs. Control group ( n = 30 controls and 52 MPN patients). B ELISA measurements demonstrated serum levels of HMGB1 in MPN and AS patients. *** P < 0.0001 vs. Control group ( n = 30 controls, 52 MPN patients, and 30 AS patients)
Article Snippet: Total RNA was extracted from bone marrow mononuclear cells using TRIzol reagent (TIANGEN, #DP424, China), and its concentration was determined with a NanoDrop 2000.
Techniques: Expressing, Control, Enzyme-linked Immunosorbent Assay